ASPSCR1 Antibody

SKU: orb1256361

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Description

ASPSCR1 Antibody

Images & Validation

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Item 1 of 9

Tested Applications	IF, IHC, IP, WB
Reactivity	Human, Mouse, Rat
Application Notes	WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).
Target	ASPSCR1
Molecular Weight	Observed: 80kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

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Tether containing UBX domain for GLUT4, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17, UBX domain-containing protein 9, ASPSCR1, ASPL, RCC17, TUG, UBXD9, UBXN9

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Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody (orb1256361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of A-549 cells, using 3 ug ASPSCR1 antibody (orb1256361). Western blot was performed from the immunoprecipitate using ASPSCR1 antibody (orb1256361) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

ASPSCR1

UniProt

Q9BZE9

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Immunofluorescence

Immunoprecipitation