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ATF4 Rabbit Polyclonal Antibody
SKU: orb389651
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | ICC, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:200-1500, IHC-P: 1:100-600, IF/ICC: 1:100-600 |
| Reactivity | Guinea pig, Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human ATF4. Please contact us for the exact immunogen sequence. The peptide is available as orb14332. |
| Target | ATF4 |
| Molecular Weight | 39 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC-P staining of rat lung tissue using ATF4 antibody (2.5 ug/ml)
Immunofluorescence image of guinea pig rectum tissue using anti-ATF4 (2.5 ug/ml)
WB analysis of mouse brain (lane 1), rat rectum (lane 2), hela cells (lane 3), u251 cells (lane 4), human breast tumour (lane 5), human lung cancer (lane 6) using anti-ATF4 (1 ug/ml)
IHC-P staining of human lung cancer tissue using ATF4 antibody (2.5 ug/ml)
Immunofluorescence analysis of guinea pig rectum tissue using anti-ATF4 (2.5 ug/ml)
IHC-P image of guinea pig rectum tissue using anti-ATF4 (2.5 ug/ml)
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
ATF4 Rabbit Polyclonal Antibody (orb389651)
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