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ATP6V1B1 Antibody (Center)
Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, C. elegans, Drosophila, Gallus, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 56833 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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All lanes: Anti-ATP6V1B1 Antibody (Center) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ATP6V1B1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ATP6V1B1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of ATP6V1B1 Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Protocol Information
ATP6V1B1 Antibody (Center) (orb1938126)
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