Aurora B Recombinant Rabbit Monoclonal Antibody

SKU: orb1152007

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Description

Images & Validation

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Item 1 of 5

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
Reactivity	Human, Mouse
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	G6D12
Immunogen	KLH conjugated synthetic peptide derived from human Aurora B
Target	AURKB
Molecular Weight	39 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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STK-1; serine/threonine kinase 12; aurora-B; aurora-1; aurora kinase B-Sv1; aurora kinase B-Sv2; ARK-2; STK-1; STK1; aurora-related kinase 2; aurora/IPL1-related kinase 2; serine/threonine-protein kinase aurora-B; aurora-and Ipl1-like midbody-associated protein 1; AURKB; AIK2; AIM-1; AIM1; ARK2; AurB; aurkb-sv1; aurkb-sv2; IPL1; STK12; STK5; AIRK2; AURKB_HUMAN.

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Aurora B Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L, (HRP), Lysate: 1: HeLa, 2: 293, 3: A20, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 39 kDa, Observed MW: 39 kDa.

Cell line: A549, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152007.

Sample: Lane 1: Human Hela cell Lysates, Lane 2: Human 293T cell Lysates, Primary: Anti-Aurora B (orb1152007) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39kDa, Observed band size: 35kDa.

Tissue: Human tonsil, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1152007.

Tissue: Mouse spleen, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1152007.

Quick Database Links

Gene Symbol

AURKB

UniProt

Q96GD4

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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