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CD276 Antibody
SKU: orb1239244
Featured
Description
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, FC, ICC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone No. | 4H3 |
| Immunogen | B7-H3 antibody was raised against the extracellular domain of human B7-H3 |
| Target | CD276 |
| Molecular Weight | Predicted: 57 kDa Observed: 100 kDa |
| Purification | B7-H3 Antibody is supplied as protein A purified IgG1. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−B7-H3 Antibody: CD276 molecule, B7H3, B7RP-2, 4Ig-B7-H3, CD276
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Western blot analysis of B7-H3 in HEK293 cells using B7-H3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.
Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml. Green: B7-H3 Antibody [4H3] (orb1239244) Blue: DAPI staining
Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml. Green: B7-H3 Antibody [4H3] (orb1239244) Blue: DAPI staining
Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.
Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.
Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with orb1240155, orb1239243, orb1239244, orb1239247, and orb1239231 antibodies at decreasing concentrations.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001019907 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD276 Antibody (orb1239244)
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