BACE1B Antibody (Center)

SKU: orb1930252

This product can be custom made

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology, Neuroscience

Images & Validation

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Item 1 of 3

Tested Applications	IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:50-100
Reactivity	Human, Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	This BACE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the Central region of human BACE1B. Antigen Region: 169-198 aa.
Target	BACE1 (HGNC:933)
Molecular Weight	55764 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Beta-secretase 1, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, BACE1, BACE, KIAA1149

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-BACE1B Ctr Pab is used in Western blot to detect BACE1B in mouse brain tissue lysate.

Anti-BACE1B Antibody (Y184) at 1:1000 dilution + human pancreas lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

BACE1 (HGNC:933)

UniProt

P56817

RefSeq (Protein):NP_620429.1, NP_036236.1, NP_001193978.1, NP_620427.1, NP_620428.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_620429.1, NP_036236.1, NP_001193978.1, NP_620427.1, NP_620428.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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