Bax Mouse Monoclonal Antibody

SKU: orb500966

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Description

Images & Validation

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Item 1 of 5

Tested Applications	ICC, WB
Dilution range	WB=1:500-2000, ICC/IF=1:50-200
Reactivity	Human
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	3C1
Immunogen	KLH conjugated synthetic peptide derived from human Bax (31-130/192aa)
Target	BAX
Molecular Weight	21 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN.

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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (orb500966) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (orb500966) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, 293T-UV (Human) Cell Lysate at 30 ug, Primary: Anti-Bax (orb500966) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.

Sample: Lane 1: Human 293T cell lysates, Lane 2: Human HT-1080 cell lysates, Lane 3: Human Raji cell lysates, Lane 4: Human SH-SY5Y cell lysates, Primary: Anti-Bax (orb500966) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 21 kDa, Observed band size: 20 kDa.

Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (orb500966) 1:100, 90 minutes at 37°C, followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

BAX

UniProt

Q07812

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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