Recombinant Bcl-2 Antibody

SKU: orb1151768

Description

Mouse monoclonal antibody to Bcl-2

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG2a, kappa
Clone No.	rBCL2/6418
Immunogen	Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for the recombinant Bcl-2 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Western blot testing of human HDLM-2 cell lysate with recombinant Bcl-2 antibody. Predicted molecular weight ~26 kDa.

IHC staining of FFPE human tonsil tissue with recombinant Bcl-2 antibody (clone rBCL2/6418). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Bcl-2 antibody (rBCL2/6418) as confirmation of integrity and purity.

Quick Database Links

UniProt

P10415

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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