You have no items in your shopping cart.
Bcl-2 Mouse Monoclonal Antibody
SKU: orb608110
Featured
Description
Research Area
Apoptosis, Apoptosis Marker, Bcl-2 Family, Cancer Research, Cell Biology, Cell Cycle, Hypoxia, Metabolism, Mitochondrial, Mitochondrial Markers
Images & Validation
−Item 1 of 3
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, Flow-Cyt=1:50-100 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b,k |
| Clone No. | 4H1 |
| Immunogen | KLH conjugated synthetic peptide derived from human Bcl-2 (51-150/239aa) |
| Target | BCL2 |
| Molecular Weight | 26 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.
Similar Products
−- Item 1 of 9
Bcl-2 Mouse Monoclonal Antibody [orb499778]
ICC, IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Sheep
Human, Rat
Mouse
Monoclonal
Unconjugated
200 μg, 200 μl, 50 μl, 100 μl - Item 1 of 7
Bcl-2 Mouse Monoclonal Antibody [orb499777]
IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Sheep
Human, Rat
Mouse
Monoclonal
Unconjugated
200 μg, 200 μl, 50 μl, 100 μl - Item 1 of 5
Bax Mouse Monoclonal Antibody [orb500966]
ICC, WB
Mouse, Rat
Human
Mouse
Monoclonal
Unconjugated
50 μl, 100 μl, 200 μl, 200 μg - Item 1 of 5
- Item 1 of 4
Bcl-2 Mouse mAb Antibody [orb763406]
IF, IHC, WB
Human, Mouse, Rat
Monoclonal
Unconjugated
100 μl, 50 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Blank control: Ctll-2. Primary Antibody (green line): Mouse Anti-Bcl-2 antibody (orb608110), dilution: 1:100, Secondary Antibody: Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Ctll-2. Primary Antibody (green line): Mouse Anti-Bcl-2 antibody (orb608110), dilution: 1:100, Secondary Antibody: Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: LOVO (Human) Cell Lysate at 30 ug, Primary: Anti-Bcl-2 (orb608110) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 26 kD.
Quick Database Links
Gene Symbol
BCL2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
Bcl-2 Mouse Monoclonal Antibody (orb608110)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days