Bcl-2 Recombinant Rabbit Monoclonal Antibody

SKU: orb612229

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Description

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Tested Applications	WB
Dilution range	WB=1:1000-5000
Reactivity	Human
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	5F7
Immunogen	A synthesized peptide derived from human Bcl 2 (48-81aa)
Target	BCL2
Molecular Weight	26 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.

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Bcl-2 Recombinant Rabbit Monoclonal Antibody

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-Bcl-2 antibody (orb612229), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human Jurkat cell lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human THP-1 cell lysates, Primary: Anti-Bcl-2 (orb612229) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26 kDa, Observed band size: 26 kDa.

Quick Database Links

Gene Symbol

BCL2

UniProt

P10415

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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