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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,κ |
| Immunogen | This BCL10 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-143 amino acids from the human region of human BCL10. Antigen Region: 1-143 aa. |
| Target | BCL10 {ECO:0000303|PubMed:9989495, ECO:0000312|HGNC:HGNC:989} |
| Molecular Weight | 26252 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−B-cell lymphoma/leukemia 10, B-cell CLL/lymphoma 10, Bcl-10, CARD-containing molecule enhancing NF-kappa-B, CARD-like apoptotic protein, hCLAP, CED-3/ICH-1 prodomain homologous E10-like regulator, CIPER, Cellular homolog of vCARMEN, cCARMEN, Cellular-E10, c-E10, Mammalian CARD-containing adapter mol
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Immunohistochemical analysis of paraffin-embedded M. kidney section using BCL10 Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. kidney section using BCL10 Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells using BCL10 Antibody (green) compared to an isotype control of mouse IgG1 (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from Daudi, Raji cell line (from left to right), using BCL10 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Quick Database Links
Gene Symbol
BCL10 {ECO:0000303|PubMed:9989495, ECO:0000312|HGNC:HGNC:989}
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
BCL10 Antibody (orb1788421)
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