BCMA Rabbit Polyclonal Antibody

SKU: orb4680

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Description

Research Area

Anti-Viral Signaling, Cancer Research, Growth Factors, Immunology

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human BCMA (12-110/184aa)
Target	TNFRSF17
Molecular Weight	27 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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B cell maturation antigen; B Cell Maturation Factor; B cell maturation protein; BCM; TNFRSF17; CD269; CD269 antigen; Tumor Necrosis Factor Receptor Member 17; Tumor necrosis factor receptor superfamily member 17; TNR17_HUMAN.

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Black line: Positive blank control (Raji), Negative blank control (HL60), Green line: Primary Antibody (Rabbit Anti-BCMA antibody (orb4680)), Orange line: Isotype Control Antibody (Rabbit IgG). Blue line: Secondary Antibody (Goat anti-rabbit IgG-PE)/ (Goat anti-rabbit IgG-AF488), Raji (Positive) and HL60 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BCMA Antibody (orb4680) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Blank control: Raji (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-BCMA antibody (orb4680), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Lane 3: Spleen (Rat) Lysate at 40 ug, Primary: Anti-BCMA (orb4680) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 27 kD.

Quick Database Links

Gene Symbol

TNFRSF17

UniProt

Q02223

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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