Berberine hydrogen sulphate

SKU: orb1221932

Description

Berberine sulfate, an alkaloid extracted from several plants, possesses antimicrobial activity against a wide variety of microorganisms including Gram-positive and Gram-negative bacteria, fungi, and protozoa.

Images & Validation

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Key Properties

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CAS Number	633-66-9
MW	433.43
Purity	>98% (HPLC)
Formula	C₂₀H₁₉NO₈S
SMILES	COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC5=C(C=C4CC3)OCO5)OC.OS(=O)(=O)[O-]
Target	Antibacterial
Solubility	In Vitro: H2O : 50 mg/mL (115.36 mM)

Bioactivity

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In Vivo

Animal model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts. Dosage: 10, 30, or 50 mg/kg/day. Administration: Gastrointestinal gavage; for 10 consecutive days. Result: Showed inhibitory rates of 33.1% and 45.3% at doses of 30 and 50 mg/kg/day.

In Vitro

Cell Proliferation Assay Cell line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29. Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM Incubation time: 72 hours. Result: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116). Cell Proliferation Assay Cell line: Colorectal carcinoma cell lines LoVo. Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM Incubation time: 24, 48, 72 hours. Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells. Cell Cycle Analysis Cell line: LoVo cells. Concentration: 0, 10, 20, 40, or 80 μM. Incubation time: 24 hours. Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population. Western blot analysis. Cell line: LoVo cells. Concentration: 10, 20, 40, or 80 μM. Incubation time: 24 hours. Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Antibacterial

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