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Berberine

SKU: orb1302341

Description

Berberine (Umbellatine) is a bioactive alkaloid known to activate AMPK, inhibit topoisomerase, and induce ROS. Its broad research applications include studying anti-tumor, antimicrobial, and metabolic effects in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research, Molecular Biology, Protein Biochemistry, Signal Transduction

Images & Validation

Key Properties

CAS Number2086-83-1
MW336.36
Purity99.67%
FormulaC20H18NO4
SMILESO(C)C1=C2C=[N+]3C(C=4C(=CC5=C(C4)OCO5)CC3)=CC2=CC=C1OC
TargetDNA/RNA Synthesis,Topoisomerase,Antibiotic,ROS,Antibacterial,Akt,Reactive Oxygen Species,PI3K,Parasite,NF-κB,Apoptosis,Endogenous Metabolite,Caspase,Autophagy,JNK
Solubility10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.05 mg/mL (6.09 mM);Chloroform, Dichloromethane, Ethyl Acetate, Acetone, etc.:Soluble;DMSO:9.8 mg/mL (29.14 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

Target IC50
LX-2 cells:> 50 μM|Hep 3B2:> 50 μM|HUVECs:18.34 μM|HeLa229 cells:42.93 μM|3T3-L1 cells:21.84 μM|Vero cells:71.14 μg/mL|HepG2 cells:> 50 μM|SW1990 cells:18.34 μM|HeLa cells:12.08 μg/mL|SMMC-7721 cells:18.34 μM|Huh7 cells:> 50 μM
In Vivo
METHODS: To test the antitumor activity in vivo, Berberine (5-10 mg/kg) was injected intraperitoneally every two days for thirty days into nude mice bearing NPC tumor C666-1. RESULTS: Berberine effectively inhibited the tumorigenicity and growth of EBV-positive NPC cell line (C666-1) in nude mice. Inhibition of tumorigenic growth of NPC cells in vivo correlated with effective inhibition of STAT3 activation in NPC cells within tumor xenografts in nude mice.
In Vitro
METHODS: Human osteosarcoma cells MG-63 were treated with Berberine (20-80 µM) for 12-24 h. Cell viability was measured by MTT assay. RESULTS: Berberine induced a concentration- and time-dependent decrease in MG-63 cell viability. METHODS: The NPC cell line HONE1 was treated with Berberine (25-50 µM) for 24 h, and the expression levels of target proteins were measured by Western Blot. RESULTS: Berberine effectively inhibited the level of p-STAT3 in HONE1 cells, and the down-regulation of STAT3 activation was associated with the inhibition of Mcl-1 expression and an increase in cleaved PARP-1 levels.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Natural Yellow 18, Natural Yellow18, Natural Yellow-18, inhibit, Inhibitor, Endogenous Metabolite, EndogenousMetabolite, Berberin, Berberine, Bacterial, Autophagy, Antibacterial, Antibiotic, Reactive Oxygen Species, ReactiveOxygenSpecies, Topoisomerase, Umbellatine

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Key Properties

No computed properties available.

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Berberine (orb1302341)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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5 mg
$ 70.00
10 mg
$ 80.00
1 ml x 10 mM (in DMSO)
$ 90.00
25 mg
$ 100.00
50 mg
$ 130.00
100 mg
$ 170.00
500 mg
$ 320.00
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