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Beta-Actin Rabbit Polyclonal Antibody (Loading Control)
SKU: orb500817
Featured
Description
Research Area
Cancer Biology; Musculoskeletal & Connective Tissue Research
Images & Validation
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Item 1 of 5
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:5000-50000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:100-500, IF=1:200-1000, Flow-Cyt=1μg/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Canine, Feline, Fish, Gallus, Guinea pig, Hamster, Insect, Porcine, Rabbit, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic MAP peptide derived from human beta-Actin (1-200/375aa) |
| Target | ACTB |
| Molecular Weight | 42 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
1 ResultsDilution
Sample
Summary
WB
Mouse
-
C2C12 myotube lysates
Lysates were centrifuged, and protein concentration...
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Bact; actin; BKRNS; BNS; BRWS1; CSMH; DDS1; PS1TP5BP1; THC8; Actx; E430023M04Rik; beta-actin; ACT-5; ACTB_CHICK; ACTB; 3.6.4.-; ACTB_BOVIN; ACTB_HORSE; ACTB_HUMAN; ACTB_MOUSE; ACTB_RABIT; ACTB_RAT; ACTB_SHEEP; actin beta; β-actin
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Quick Database Links
Gene Symbol
ACTB
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Filter by Applications
Filter by Species
Min-Wook Hong 1,Hun Kim 1,So-Young Choi 1,Neelesh Sharma 2,*ORCID andSung-Jin Lee Effect of Gossypol on Gene Expression in Swine Granulosa Cells toxins, (2024)
Applications
WB
Reactivity
Porcine
Fatemeh Imanparast, Mohammad Ali Faramarzi, Akram Vatannejad, Maliheh Paknejad, Behnas Deiham, Farzad Kobarfard, Amir Amani, Mahmood Doosti mZD7349 peptide-conjugated PLGA nanoparticles directed against VCAM-1 for targeted delivery of simvastatin to restore dysfunctional HUVECs Microvascular Research, 112, 14 (2017)
Applications
WB
Reactivity
Human
Al-Yhya, Nouf et al. Pharmacological inhibition of HDAC1/3-interacting proteins induced morphological changes, and hindered the cell proliferation and migration of hepatocellular carcinoma cells Environ Sci Pollut Res Int, (2021)
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days