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Beta-arrestin 2 Rabbit Polyclonal Antibody
SKU: orb526755
Featured
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Bovine, Equine, Human, Porcine, Rabbit, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Beta-arrestin 2 (251-350/409aa) |
| Target | ARRB2 |
| Molecular Weight | 45 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−beta-arrestin 2; beta Arrestin 2; ARB 2; ARB2; ARR 2; ARR2; ARRB 2; ARRB2; ARRB2_HUMAN; Arrestin 3; Arrestin beta 2; Arrestin beta-2; BARR2; DKFZp686L0365; HGNC:712; beta-arrestin-2.
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25 ug total protein per lane of various lysates probed with Beta-arrestin 2 polyclonal antibody, unconjugated (orb526755) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Beta-arrestin 2) polyclonal Antibody, Unconjugated (orb526755) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beta-arrestin 2) Polyclonal Antibody, Unconjugated (orb526755) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Quick Database Links
Gene Symbol
ARRB2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Beta-arrestin 2 Rabbit Polyclonal Antibody (orb526755)
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