beta-Catenin Antibody

SKU: orb153335

Description

Goat polyclonal antibody to Catenin (cadherin-associated protein), beta 1. Beta Catenin is a 88 kDa protein and is a key downstream effector in the Wnt signalling pathway. It is involved in early embryonic development and tumorigenesis. Beta Catenin is part of a complex of proteins that constitute adherens junctions, necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. This protein anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete.

Research Area

Signal Transduction

Images & Validation

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Tested Applications	IF, IHC-P, WB
Dilution Range	WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:250-1:1,000
Reactivity	Canine, Human, Monkey, Mouse, Rat
Application Notes	The antibody solution should be gently mixed before use.

Key Properties

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Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Antigen: Purified recombinant peptide derived from within residues 731 aa to the C-terminus of human beta Catenin produced in E. coli. Antigen Sequence: MDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL
Target	Catenin (cadherin-associated protein), beta 1, 88kDa
Purification	Epitope affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration	Please inquire
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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cadherin-associated protein, CTNNB, CTNNB1, MRD19, armadillo antibody.

Beta catenin Rabbit Polyclonal Antibody [orb500798]

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep, Zebrafish
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

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beta Catenin/CTNNB1 Rabbit Polyclonal Antibody [orb402308]
Applications:
ELISA, FC, ICC, IF, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg

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Beta catenin Rabbit Polyclonal Antibody [orb10246]

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep, Zebrafish
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

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CTNNB1 Antibody (C-term) [orb1927592]
Applications:
FC, IF, IHC-P, WB
Predicted Reactivity:
Mouse, Rat, Zebrafish
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl

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beta-Catenin Antibody at 1/1,000 dilution, lysate at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.

Confocal immunofluoroscence - beta-Catenin Antibody in BEAS-2B cells at 1/250 dilution, cells were fixed with 4% of PFA.

IHC of mouse intestine using beta-Catenin Antibody and FFPE tissue after heat-induced antigen retrieval. beta-Catenin Antibody at 1:500/DAB detection.

IHC of mouse liver using beta-Catenin Antibody and FFPE tissue after heat-induced antigen retrieval. beta-Catenin Antibody at 1:500/DAB detection.

IHC of mouse brain using beta-Catenin Antibody and FFPE tissue after heat-induced antigen retrieval. beta-Catenin Antibody at 1:500/DAB detection.

IHC of mouse lung using beta-Catenin Antibody and FFPE tissue after heat-induced antigen retrieval. beta-Catenin Antibody at 1:500/DAB detection.

IHC of mouse retina using beta-Catenin Antibody and FFPE tissue after heat-induced antigen retrieval. beta-Catenin Antibody at 1:500/DAB detection.

Quick Database Links

Gene Symbol

Catenin (cadherin-associated protein), beta 1, 88kDa

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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