Recombinant Beta Catenin Antibody / Rabbit Monoclonal

SKU: orb2640858

Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. [RefSeq]

Images & Validation

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Tested Applications	IF, IHC-P, WB
Dilution Range	Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Prediluted IHC only format : incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	Titration of the recombinant beta Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	CTNB1-1R
Immunogen	Beta Catenin protein was used as the immunogen for the recombinant beta Catenin antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Beta Catenin Antibody / Rabbit Monoclonal

Immunofluorescent staining of human HeLa cells with recombinant Beta Catenin antibody (clone CTNB1-1R, green) and Reddot nuclear stain (red).

Western blot testing of human HeLa antibody with recombinant Beta Catenin antibody (clone CTNB1-1R). Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

IHC staining of FFPE human breast cancer tissue with recombinant beta Catenin antibody (clone CTNB1-1R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

UniProt

P35222

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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