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Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Tubulin-Beta (31-130/444 aa) |
| Target | Beta Tubulin |
| Molecular Weight | 50 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Beta 4 tubulin; Tubulin-beta; Tubulin beta; Beta 5 tubulin; BetaTubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain.
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Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Beta Tubulin (orb11537) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.
Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Beta Tubulin (orb11537) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.
Sample: Lane 1: Cerebellum (Rat) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Ovary (Mouse) Lysate at 40 ug, Lane 6: Heart (Mouse) Lysate at 40 ug, Lane 7: Heart (Rat) Lysate at 40 ug, Primary: Anti-beta Tubulin (orb11537) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.
Quick Database Links
Gene Symbol
Beta Tubulin
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Filter by Applications
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Rituparna Ghosh 1, Biswadev Bishayi 2 Endogenous blocking of TLR2 along with TNF-α and IL-1β ameliorates the severity of the S. aureus arthritis via modulating STAT3/SOCS3 expressions in tissue resident macrophages Microb Pathog ., (2024)
Applications
WB
Reactivity
Mouse
Sharmistha Samanta , Gopinath Mukherjee , Sreya Chattopadhyay , Biswadev Bishayi * Concomitant inhibition of TLR4 and NLRP3 inflammasome in macrophages regulates lipopolysaccharide induced inflammation The Microbe, (2025)
Applications
WB
Reactivity
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Gopinath Mukherjee et al. TREATMENT OF MICE WITH TLR4 AND IFN-γ ANTIBODY AND EXOGENOUS IL-10 MODULATES BCL2 AND LC3 EXPRESSION IN RANKL/M-CSF PLUS LPS STIMULATED BONE MARROW MACROPHAGES-LEADING TO BONE RESORPTION researchgate.net, (2025)
Applications
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Reactivity
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Beta Tubulin Rabbit pAb (orb11537)
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