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Biotin-14-dCTP

SKU: orb533284

Description

Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dCTP or Biotin-16-dCTP are recommended whose Biotin moiety is attached to C5 position of cytidine via a 11-atom or 16-atom linker, respectively. Recommended Biotin-14-dCTP/dCTP ratio for Nick Translation: 50% Biotin-14-dCTP/50% dCTP.

Images & Validation

Application Notes
Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment.
Spectroscopic Propertie: λmax 272 nm, ε 13.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

MWTheoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
Purity≥ 95% (HPLC)
FormulaC31H54N7O16P3S
SMILESOP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2ccc(nc2=O)NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23)C[C@@H]1O

Customer Validated Data

The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution

Latest Experiments

5 Results
Dilution
Sample
Summary
DNA end-labeling (Micro-C assay)
Drosophila melanogaster
200 μM
Early embryos of Drosophila melanogaster (nuclear cycle 14)
Biotin-14-dCTP is used as part of...
Chromatin conformation capture assay
Drosophila melanogaster, Salmonella enterica
200 μM
Chromatin or genomic DNA derived from fixed cells (e.g., embryos or bacterial cultures)
Biotin-14-dCTP is used as a labeling...
DNA biotinylation
In vitro single-molecule system
33 µM
In vitro assembled transcription elongation complex (EC) on DNA scaffold
Biotin-14-dCTP was used to generate terminally...
Enzymatic DNA end-labeling
In vitro(cell-free)
33 μM
Purified linearized plasmid DNA
Biotin-14-dCTP is used for enzymatic DNA...
Klenow fill-in end-labeling of λ DNA
In vitro(cell-free)
33 μM
Linear double-stranded bacteriophage λ DNA and histones from S. cerevisiae
Biotin-14-dCTP is used for end-labeling DNA...

Storage & Handling

Storagestore at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Bio-14-dCTP, Triethylammonium salt

Similar Products

  • Biotin-14-dCTP [orb1733736]

    ≥ 95% (HPLC)

    Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)

    C31H54N7O16P3S

    200 μl
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Key Properties

No computed properties available.

Protocol Information

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5 x 200 μl
$ 740.00
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