Recombinant Human 4-1BB Protein (Biotin)

SKU: orb2993093

Description

Biotinylated Recombinant Human 4-1BB Ligand Receptor is produced by our Mammalian expression system and the target gene encoding Leu24-Gln186 is expressed with a Avi, 6His tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human cells
Biological Origin	Human
Biological Activity	BLI: Loaded Biotinylated Human 4-1BB-Avi-6His(Cat#orb2993093) on SA Biosensor, can bind Human 4-1BBL-His(Cat#orb2994593) with an affinity constant of -3 nM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human 4-1BB-Avi-6His(Cat#orb2993093) on SA Biosensor, can bind Anti-Human CD137 mAb-Fc(Cat#orb2993114) with an affinity constant of -3 nM as determined in BLI assay. (Regularly tested)
Tag	C-Avi-6His
Molecular Weight	Predicted: 19.9 KDa. Observed: 27-35 KDa, reducing conditions
Expression Region	Leu24-Gln186
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA

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UniProt

Q07011

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No UniProt data available

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