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Biotinylated Human B7-2
SKU: orb2993754
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Human CTLA-4-Fc(Cat#orb2994544) on Protein A Biosensor, can bind Human B7-2-His-Avi(Cat#orb2993754) with an affinity constant of 0.37 uM as determined in BLI assay. (Regularly tested) |
| Tag | C-Avi-6His |
| Molecular Weight | Predicted: 28.4 KDa. Observed: 55-80 KDa, reducing conditions |
| Expression Region | Ala24-Pro247 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−T-Lymphocyte Activation Antigen CD86; Activation B7-2 Antigen; B70; BU63; CTLA-4 Counter-Receptor B7.2; FUN-1; CD86; CD28LG2
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−Biotinylated Recombinant Human CD86/B7-2 Protein, Avi His Tag [orb1550588]
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
Biotinylated Recombinant Human CD86/B7-2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Leu26-Pro247) of Human CD86/B7-2 fused with a His Tag and Avi tag at the C-terminal.
100 μg
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Biotinylated Human B7-2 (orb2993754)
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