Recombinant Human FcRn Heterodimer Protein (Biotin)

SKU: orb2993881

Description

Biotinylated Recombinant Human IgG Fc fragment receptor transporter is produced by our Mammalian expression system and the target gene encoding Ala24-Ser297&Ile21-Met119 is expressed with a 6His, Avi tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	BLI: Loaded Biotinylated Human FcRn Heterodimer-His-Avi(Cat#orb2993881) on HIS1K Biosensor, can bind Anti-Human HER2 mAb (Human IgG1) (Cat#GMP-A062) with an affinity constant of 0.11 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human FcRn Heterodimer (C-6His-Avi) (Cat#orb2993881) on HIS1K Biosensor, can bind Anti-Human CD137 mAb (Human IgG4) (Cat#orb2993114) with an affinity constant of 0.66 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human FcRn Heterodimer (C-6His-Avi) (Cat#orb2993881) on HIS1K Biosensor, can bind Recombinant Human IgG1 Fc (Cat#orb2993960) with an affinity constant of 0.254 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human FcRn Heterodimer (C-6His-Avi) (Cat#orb2993881) on HIS1K Biosensor, can bind Recombinant Human IgG1 Fc (Cat#orb2992939) with an affinity constant of 0.588 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human FcRn Heterodimer (C-6His-Avi) (Cat#orb2993881) on HIS1K Biosensor, can bind Recombinant Human IgG2 Fc (223AA) (Cat#orb2993943) with an affinity constant of 0.251 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human FcRn Heterodimer (C-6His-Avi) (Cat#orb2993881) on HIS1K Biosensor, can bind Recombinant Human IgG2 Fc (228AA) (Cat#orb2993938) with an affinity constant of 0.436 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human FcRn Heterodimer (C-6His-Avi) (Cat#orb2993881) on HIS1K Biosensor, can bind Recombinant Human IgG3 Fc (Cat#orb2993894) with an affinity constant of 0.117 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human FcRn Heterodimer (C-6His-Avi) (Cat#orb2993881) on HIS1K Biosensor, can bind Recombinant Human IgG4 Fc (228AA) (Cat#orb2993937) with an affinity constant of 0.421 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human FcRn Heterodimer (C-6His-Avi) (Cat#orb2993881) on HIS1K Biosensor, can bind Recombinant Human IgG4 Fc (224AA) (Cat#orb2993942) with an affinity constant of 1.07 uM as determined in BLI assay. (Regularly tested)
Tag	C-6His-Avi
Molecular Weight	Predicted: 31.1&13.5 KDa. Observed: 35&13 KDa, reducing conditions
Expression Region	Ala24-Ser297&Ile21-Met119
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC.(QC verified)
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 7.8.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IgG receptor FcRn; Neonatal Fc receptor; FCRN

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UniProt

P61769

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No UniProt data available

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