Recombinant Human Siglec-15 Protein (Biotin)

SKU: orb2993902

Description

Biotinylated Recombinant Human Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	BLI: Loaded Biotinylated Human Siglec-15-Fc(at#orb2993902) on AHC Biosensor, can bind Anti-Human Siglec15 mAb-mFc(Cat#orb2993156) with an affinity constant of 0.13 nM as determined in BLI assay. (Regularly tested)
Tag	C-Fc-Avi
Molecular Weight	Predicted: 55.3 KDa. Observed: 58-75 KDa, reducing conditions
Expression Region	Phe20-Thr263
Purity	SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified)
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, 150mM NaCl, 0.3% Chaps, 5% Trehalose, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3; CD33L3

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Q6ZMC9

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