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Recombinant Human TGF-beta 3 Protein (Biotin)
SKU: orb2993088
Description
Images & Validation
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Key Properties
−| Expression System | Human cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human TGFBR2-Fc(Cat#orb3002421) at 2μg/ml (100 μl/well)can bind Biotinylated Human TGF-beta 3-Avi (Cat#orb2993088). The ED50 of Biotinylated Human TGF-beta 3-Avi (Cat#orb2993088)is 2.05 ng/ml.. Cell Based Assay: Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells.The ED50 for this effect is 0.37ng/ml. |
| Tag | N-Avi |
| Molecular Weight | Predicted: 14.5 KDa. Observed: 10-14 KDa, reducing conditions |
| Expression Region | Ala301-Ser412 (Tyr340Phe) |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 4mM HCl. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TGFB3; ARVD; TGF-beta3
Similar Products
−Recombinant Human TGF-beta 3 Protein (Biotin) [orb2993078]
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 12.7&35.3 KDa. Observed: 12&37-45 KDa, reducing conditions
20 μg, 100 μg
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Recombinant Human TGF-beta 3 Protein (Biotin) (orb2993088)
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