Recombinant Human TROP-2 Protein (Biotin)

SKU: orb2993745

Description

Biotinylated Recombinant Human Tumor-associated Calcium Signal Transducer 2 is produced by our Mammalian expression system and the target gene encoding His27-Thr274 is expressed with a Avi, 6His tag at the N-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Biotinylated Human TROP-2-Avi-His(Cat#orb2993745) at 1μg/ml (100 μl/well) can bind Anti-Human TROP-2 mAb(Cat#orb2993115).The ED50 of Anti-Human TROP-2 mAb(Cat#orb2993115) is 4.19 ng/ml.. BLI: Loaded Anti-Human TROP-2 mAb-Fc(Cat#orb2993115) on Protein A Biosensor, can bind Biotinylated Human TROP-2-Avi-His(Cat#orb2993745) with an affinity constant of 0.49 nM as determined in BLI assay.
Tag	C-Avi-6His
Molecular Weight	Predicted: 30.5 KDa. Observed: 40-60 KDa, reducing conditions
Expression Region	His27-Thr274
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tumor-associated calcium signal transducer 2; Membrane component chromosome 1 surface marker 1; Cell surface glycoprotein Trop-2; TACSTD2; TROP2

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UniProt

P09758

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No UniProt data available

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