Bleomycin hydrochloride

SKU: orb1688869

Description

Bleomycin sulfate is a glycopeptide antibiotic that inhibits DNA synthesis and induces DNA strand scission, exhibiting antitumor properties. It is widely used in cancer research for studying DNA damage mechanisms and chemotherapeutic effects in both cellular and animal models.

Research Area

Cell Biology, Molecular Biology

Images & Validation

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Key Properties

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CAS Number	67763-87-5
MW	1451.01(Average)
Purity	98.00% (May vary between batches)
Formula	C₅₅H₈₄ClN₁₇O₂₁S₃
SMILES	[Cl-].C[C@@H](O)[C@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@@H](NC(=O)c1nc(nc(N)c1C)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]1O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]1O[C@H]1O[C@H](CO)[C@@H](O)[C@H](OC(N)=O)[C@@H]1O)c1cnc[nH]1)C(=O)NCCc1nc(cs1)-c1nc(cs1)C(=O)NCCC[S+](C)C
Target	DNA/RNA Synthesis
Solubility	H2O:80 mg/mL;10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL;DMSO:255 mg/mL

Bioactivity

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Target IC50	UT-SCC-12A cells:14.2 ± 2.8 nM\|UT-SCC-12B cells:13.0 ± 1.1 nM\|UT-SCC-19A cells:4.0 ± 1.3 nM
In Vivo	METHODS: To detect the anti-tumor activity in vivo, Bleomycin Sulfate (20 mg/kg) was intraperitoneally injected into BALB/c mice carrying mouse colorectal cancer tumor CT26 shControl or shCRT, once every two days for five times. RESULTS: Bleomycin Sulfate delayed the growth of CT26 cells transfected with control shRNA, but had no effect on CT26 cells transfected with CRT shRNA. METHODS: To study the time course of Bleomycin induced pulmonary fibrosis in mice, Bleomycin Sulfate (0.06 mg/ mouse in 0.9% saline) was administered as a single intratracheal instillation (IT) to C57Bl/6J mice. RESULTS: Bleomycin Sulfate induced pulmonary fibrosis in mice. The most appropriate time point for evaluating pulmonary fibrosis in this model is 14 days after IT infusion of Bleomycin Sulfate. This is based on the observation that extensive fibrosis occurred in animals at 14 days, but the variability of the fibrotic response was smaller and the mortality rate was lower than 21 days. Read more...
In Vitro	METHODS: Human pancreatic cells (PCCs) AsPC-1 and MIA PaCa-2 were treated with Bleomycin Sulfate (0.1-100 µM) for 24-72 hours, and the cell growth inhibition was detected by the MTT method. RESULTS: AsPC-1 cells showed no response to Bleomycin Sulfate. Bleomycin Sulfate significantly inhibited the cell growth of MIA-PaCa-2, and the IC50 at 24/48/72 h was 5.9/6.4/2.6 μM, respectively. METHODS : Mesenchymal stem cells (MSCs) were treated with Bleomycin Sulfate (1800 ng/mL) for 24-96 hours, and sub-G1 and caspase-3 were detected by Flow Cytometry. RESULTS: Bleomycin Sulfate induces upregulation of sub-G1 and caspase-3 activation, indicating that Bleomycin Sulfate induces apoptosis. METHODS : Normal rat alveolar macrophages were incubated with Bleomycin Sulfate (0.01-1 μg/mL) for 18 hours, and their levels were determined using the MDGF Assay. RESULTS: Bleomycin Sulfate stimulates macrophages to produce fibroblast growth factor (MDGF). Read more...

Storage & Handling

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Storage	store at low temperature,keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DNA synthesis, DNA Synthesis, DNASynthesis, Bleomycin Hydrochloride, Bleomycinhydrochloride, RNASynthesis, RNA Synthesis

Quality Guarantee

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Compound Identifiers

PubChem CID

76960929 ↗

Key Properties

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No computed properties available.

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