Cart summary

You have no items in your shopping cart.

BOT-64

SKU: orb1298697

Description

BOT-64 is a cell-permeable benzoxathiole compound that acts as an IKK-2 inhibitor by targeting the Ser177 and Ser181 residues in its activation loop. This small molecule is used in research to study NF-κB signaling in cellular and animal models of inflammation and immune response.

Research Area

Signal Transduction

Images & Validation

Key Properties

CAS Number113760-29-5
MW273.35
Purity99.73%
FormulaC15H15NO2S
SMILESCC1(C)Cc2oc(=Nc3ccccc3)sc2C(=O)C1
TargetIκB/IKK
SolubilityDMSO:30 mg/mL (109.75 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.32 mM)

Bioactivity

Target IC50
IKKβ:1 μM
In Vitro
BOT-64 inhibits IKKbeta-mediated IkappaBalpha phosphorylation in LPS-activated macrophages, resulting in sequential prevention of downstream events, including proteolytic degradation of IkappaBalpha, DNA binding ability, and transcriptional activity of NF-kappaB. BOT-64 inhibits LPS-inducible IKKbeta activity in the cells and catalytic activity of highly purified IKKbeta. Moreover, the effect of BOT-64 on cell-free IKKbeta was abolished by substitution of Ser-177 and Ser-181 residues in the activation loop of IKKbeta to glutamic acid residues, indicating a direct interaction site of benzoxathiole. BOT-64 attenuates NF-kappaB-regulated expression of inflammatory genes such as inducible nitric-oxide synthase, cyclooxygenase-2, tumor necrosis factor-alpha, interleukin (IL)-1beta, and IL-6 in LPS-activated or expression vector IKKbeta-transfected macrophages.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Inhibitor, I kappa B kinase, IkB, IkB/IKK, IKK, IKK2, inflammatory, IL-6, IκB kinase, IκB, inhibit, NF-κb, BOT 64, BOT64, BOT-64, RAW 264.7

Similar Products

  • BOT-64 [orb1219481]

    >98% (HPLC)

    113760-29-5

    273.35

    C15H15NO2S

    10 mg, 25 mg, 50 mg, 100 mg, 1 g, 500 mg, 200 mg, 5 mg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Key Properties

No computed properties available.

Documents Download

Datasheet
Product Information
Download

Request a Document

Protocol Information

BOT-64 (orb1298697)

  • Star
  • Star
  • Star
  • Star
  • Star
  • 0.0
Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

Login to Submit a Review

No reviews yet

Step 1: Enter information below

(Recommended: An additional animal making an allowance for loss during the experiment)

Step 2: Enter the in vivo formulation

(This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

1 mg
$ 90.00
1 ml x 10 mM (in DMSO)
$ 150.00
5 mg
$ 160.00
10 mg
$ 230.00
25 mg
$ 400.00
50 mg
$ 590.00
100 mg
$ 780.00
200 mg
$ 1,040.00
DispatchUsually dispatched within 3-5 working days
Bulk Enquiry