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BRAF35 Rabbit Polyclonal Antibody

SKU: orb155865

Description

BRAF35 Rabbit Polyclonal Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution RangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityMouse
Predicted ReactivityBovine, Human, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human BRAF35 (1-100/317aa)
TargetHMG20B
Molecular Weight36 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

BRAF25; BRAF35; HMGX2; HMGXB2; PP7706; SMARCE1r; SOXL; pp8857; HM20B_HUMAN; HMG20B; SMARCE1-related protein; BRCA2-associated factor 35; HMG box-containing protein 20B; HMG domain-containing protein 2; HMG domain-containing protein HMGX2; Sox-like transcriptional factor; Structural DNA-binding protein BRAF35;

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BRAF35 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BRAF35) Polyclonal Antibody, Unconjugated (orb155865) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

BRAF35 Rabbit Polyclonal Antibody (orb155865)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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