BRCA1 Rabbit Polyclonal Antibody

SKU: orb573638

Description

Rabbit polyclonal antibody to BRCA1

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Molecular Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	WB
Reactivity	Human
Predicted Reactivity	Canine, Equine, Rabbit, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human BRCA1
Target	BRCA1
Protein Sequence	Synthetic peptide located within the following region: MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLK
Molecular Weight	76kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IRIS, PSCP, BRCAI, BRCC1, FANCS, PNCA4, RNF53, BROVCA1, PPP1R53

Phospho-RAD51 (Thr309) Rabbit Polyclonal Antibody [orb4742]

Applications:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Gallus, Porcine, Rabbit, Rat, Sheep
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 200 μl, 50 μl, 100 μl

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Rabbit Anti-BRCA1 Antibody, Catalog Number: orb573638, Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue, Observed Staining: Cytoplasm, Primary Antibody Concentration: 1:100, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-BRCA1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: ACHN cell lysate, BRCA1 is supported by BioGPS gene expression data to be expressed in ACHN.

Quick Database Links

Gene Symbol

BRCA1

UniProt

P38398

RefSeq (Protein):NP_009229

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_009229

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Protocol Information

Western Blot (IB, immunoblot)

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