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BST1 Rabbit Polyclonal Antibody
SKU: orb100702
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB=1:500-2000, Flow-Cyt=1μg/Test |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Porcine, Rabbit, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human BST1/CD157 (51-150/318aa) |
| Target | BST1 |
| Molecular Weight | 33 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Cyclic ADP ribose hydrolase 2; ADP ribosyl cyclase 2; Bone marrow stromal antigen 1; Bone marrow stromal cell antigen 1; BST 1; BST1; BST-1; cADPr hydrolase 2; CD157; CD157 antigen; NAD(+) nucleosidase; BST1_HUMAN.
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Blank control: U937 (blue). Primary Antibody: Rabbit Anti-BST1 antibody (orb100702), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb100702, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.
Sample: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-BST1 (orb100702) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.
Quick Database Links
Gene Symbol
BST1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
BST1 Rabbit Polyclonal Antibody (orb100702)
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