BubR1 Rabbit Polyclonal Antibody

SKU: orb101245

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Description

Research Area

Cancer Research, Cell Biology, Cell Cycle, Phosphatases

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Rabbit

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human BubR1 (751-850/1050aa)
Target	BUB1B
Molecular Weight	120 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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beta homolg of S. cerevisiae BUB 1; beta homolg of S. cerevisiae budding uninhibited by benzimidazoles; BUB 1B; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; Bub1A; BUB1B; BUB1B_HUMAN; BUB1beta; BUBR1; Budding Uninhibited by Benzimidazoles 1 beta; hBUBR1; MAD3/BUB1 related protein kinase; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint gene BUB1B; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine protein kinase BUB1 beta; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; Protein SSK1; SSK 1; SSK1.

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Sample: HepG2 (Human) Cell Lysate at 30 ug, Hcclm3 (Human) Cell Lysate at 30 ug, Primary: Anti-BubR1 (orb101245) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Thymus (Mouse) Lysate at 40 ug, Primary: Anti-BubR1 (orb101245) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

Quick Database Links

Gene Symbol

BUB1B

UniProt

O60566

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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