C-176

SKU: orb1302045

Description

C-176 is a selective and brain-penetrant small molecule inhibitor of the STING pathway, suppressing STING-mediated IFNβ production and inflammation. It is widely used in research to study neuroinflammation, autoimmune diseases, and cancer immunotherapy in both cellular and animal models.

Research Area

Immunology & Inflammation

Images & Validation

−

Key Properties

−

CAS Number	314054-00-7
MW	358.09
Purity	99.97% (May vary between batches)
Formula	C₁₁H₇IN₂O₄
SMILES	[O-][N+](=O)c1ccc(o1)C(=O)Nc1ccc(I)cc1
Target	STING
Solubility	DMSO:120 mg/mL (335.11 mM);10% DMSO+40% PEG300+5% Tween80+45% Saline:1.8 mg/mL (5.03 mM)

Bioactivity

−

Target IC50	HCC1143 cells:9.5 μM\|RAW 264.7 cells:1.14 μM\|HCC1806 cells:6.2 μM\|HCC38 cells:8.7 μM
In Vivo	METHODS: To investigate the anti-inflammatory activity in vivo, C-176 (750/375 nmol per mouse in corn oil) was injected intraperitoneally into C57BL/6J mice, and CMA (224 mg/kg) was administered either 1 h or 4 h. After 4 h, the mice were euthanized, and the serum was collected for measurement of CMA-induced cytokine levels. RESULTS: C-176 significantly reduced the CMA-mediated induction of Type 1 IFNs and IL-6 serum levels without significant toxicity. Read more...
In Vitro	METHODS: HEK293T cells expressing mCherry-STING were transfected with plasmids encoding cdG Syn or RIG-I and an IFNβ luciferase reporter gene, then treated with C-176 (0.01-1.25 µM) to detect IFNβ luciferase activity. RESULTS: C-176 strongly reduced STING-mediated, but not RIG-I or TBK 1-mediated, IFNβ reporter activity. METHODS: The rat microglial cell line GMI-R1 was treated with LPS (1 µg/mL) and C-176 (20 µM), and target protein expression levels were measured by Western Blot. RESULTS: LPS activated the STING signaling pathway. Although the levels of mitochondrial superoxide and STING were not affected by C-176 treatment, the increase in the phosphorylation levels of TBK1 and NF-κB could be significantly reversed by C-176, suggesting that STING signaling could be inhibited by C-176 treatment in GMI-R1 cell line. Read more...

Storage & Handling

−

Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

MITA, inhibit, MPYS, ERIS, Inhibitor, C176, C-176, C 176, TMEM173, STING inhibitor 1, STING, Stimulator of Interferon Genes

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

1103958 ↗

Key Properties

−

No computed properties available.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols

C-176 (orb1302045)

Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

No reviews yet

Purity:	>98% (HPLC)
CAS Number:	314054-00-7
MW:	358.09
Formula:	C₁₁H₇IN₂O₄

C-176

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Similar Products

C176 [orb1221177]

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

Request a Document

Protocol Information

C-176 (orb1302045)

Step 1: Enter information below

Step 2: Enter the in vivo formulation

Available Sizes

Email

UK Office

US Office

CN Office

Distributors