C29

SKU: orb1304140

Description

C29 is a selective small molecule inhibitor of Toll-like receptor 2 (TLR2) heterodimer signaling. It potently blocks both TLR2/1 and TLR2/6 pathways induced by synthetic and bacterial ligands in human cell lines (HEK-TLR2, THP-1), but specifically inhibits TLR2/1 signaling in murine macrophages. This tool compound is useful for in vitro research dissecting TLR2-mediated immune responses in infection, inflammation, and autoimmunity.

Research Area

Immunology & Inflammation

Images & Validation

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Key Properties

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CAS Number	363600-92-4
MW	285.29
Purity	97.37%
Formula	C₁₆H₁₅NO₄
SMILES	COc1cccc(\C=N\c2cccc(C(O)=O)c2C)c1O
Target	TLR
Solubility	DMSO:125 mg/mL (438.15 mM);H2O:Insoluble;10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.01 mM)

Bioactivity

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In Vivo

C29L inhibits TLR2/1-induced inflammation in mice. Mice treated twice with C29L before administration of P3C significantly blocks IL-12 p40 and TNF-α liver cytokine mRNA and serum protein. C29L has a significant inhibitory effect at the later time point for IL-12 p40.

In Vitro

C29 blunts hTLR2/1 and hTLR2/6 signaling in HEK-TLR2 Stable transfectants and THP-1 Cells. C29 blocks P3C- and P2C-induced IL-8 mRNA dose-dependently in HEK-TLR2 stable transfectants. C29 also inhibits P3C- and P2C-induced IL-1β gene expression significantly at both 1 h and 4 h following stimulation, as well as both P3C- and P2C-induced NF-κB–luciferase activity in transiently transfected HEK293T cells expressing hTLR2 and an NF-κB–sensitive luciferase reporter construct. C29 preferentially inhibits TLR2/1 signaling in primary murine macrophages. C29 blocks TLR2 bacterial agonist-induced proinflammatory gene expression in HEK-TLR2 Cells and murine macrophages. C29 inhibits ligand-induced interaction of TLR2 with MyD88 and blocks MAPK and NF-κB activation.

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Toll-like Receptor (TLR), Toll-like, TLR, TLR2/1, TLR2, TLR2/6, Inhibitor, inhibit, hTLR2/1, hTLR2/6, C 29, C29, C-29

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Compound Identifiers

PubChem CID

3579893 ↗

Key Properties

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No computed properties available.

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C29 (orb1304140)

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Applications:	ELISA, WB
Reactivity:	A. thaliana
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

CAS Number:	65586-25-6
MW:	915.08
Formula:	C₄₆H₇₄O₁₈

Purity:	>98% (HPLC)
CAS Number:	363600-92-4
MW:	285.3
Formula:	C₁₆H₁₅NO₄

C29

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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C29 (orb1304140)

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