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C4A, N/A Antibody (CF488)
SKU: orb3073864
Description
Images & Validation
−
| Tested Applications | ELISA, IF, IHC |
|---|---|
| Dilution range | ELISA (For coating use Ab at 1-2ug/ml order Ab without BSA); Immunofluorescence (1:50-1:100);Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | C4D204 |
| Immunogen | Recombinant full-length human Complement 4d protein |
| Target | C4A, N/A |
| Source/Expression System | Bioreactor |
| Molecular Weight | 192kDa (predicted) |
| Purification | Protein G |
| Conjugation | CF488 |
Storage & Handling
−| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
|---|---|
| Buffer/Preservatives | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab conjugated to CF488 |
| Disclaimer | For research use only |
Alternative Names
−Acidic Complement C4; Basic C4; C4-1; C4a Anaphylatoxin; C4A; C4A2; C4A3; C4A4; C4A6; C4b; C4B1; C4B12; C4d fragment; C4F; C4S; Complement C4 Gamma Chain; Complement Component 4A; Complement Component 4B

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Protocol Information
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
C4A, N/A Antibody (CF488) (orb3073864)
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