Recombinant Carbonic Anhydrase 9 Antibody / CA9

SKU: orb2635846

Description

In normal kidney, CA9 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CA9 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Carbonic Anhydrase 9 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG2b, kappa
Clone No.	rPN-15
Immunogen	Recombinant full-length human CA9 protein was used as the immunogen for the recombinant Carbonic Anhydrase 9 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Carbonic Anhydrase 9 Antibody / CA9

IHC staining of FFPE human renal cell carcinoma tissue with recombinant Carbonic Anhydrase 9 antibody (clone rPN-15). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Carbonic Anhydrase 9 antibody (clone rPN-15) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q16790

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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