Recombinant Carbonic Anhydrase 9 Antibody

SKU: orb2642347

Description

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The optimal dilution of the recombinant Carbonic Anhydrase 9 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	CA9/2993R
Immunogen	Recombinant human protein was used as the immunogen for this recombinant Carbonic Anhydrase 9 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human renal cell carcinoma with recombinant Carbonic Anhydrase 9 antibody (clone CA9/2993R). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Carbonic Anhydrase 9 antibody (clone CA9/2993R) as confirmation of integrity and purity.

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UniProt

Q16790

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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