CALB1 Protein

SKU: orb424478

Description

Recombinant of CALB1 protein

Images & Validation

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Application Notes

Applications: CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedureA- Dilute 1 µl of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).B- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented

Key Properties

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Source	Escherichia Coli
Purity	Greater than 90% as determined by SDS-PAGE.

Storage & Handling

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Storage	Stability: Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP28K should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
Form/Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Buffer/Preservatives	The protein was lyophilized from a concentrated solution (1mg/ml) containing 50mM NaHCO3.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Calbindin, Vitamin D-dependent calcium-binding protein, avian-type, Calbindin D28, D-28K, Spot 35 protein, Calb1, CaBP28K, MGC93326.

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CALB1 Protein (orb424478)

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Applications:	ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Porcine, Rabbit
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IHC, IP, WB
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IHC, WB
Predicted Reactivity:	Bovine, Canine, Porcine
Reactivity:	Human, Rat
Species/Host:	Goat
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Porcine, Rabbit
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Human, Mouse, Rat
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Recombinant
Conjugation:	Unconjugated

CALB1 Protein

Description

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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CALB1 Protein (orb424478)

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