Calcitriol

SKU: orb1300876

Description

Calcitriol

Research Area

Metabolism Research

Images & Validation

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Key Properties

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CAS Number	32222-06-3
MW	416.64
Purity	99.94%
Formula	C₂₇H₄₄O₃
SMILES	C[C@@]12[C@](/C(=C/C=C/3\C(=C)[C@@H](O)C[C@H](O)C3)/CCC1)(CC[C@@]2([C@@H](CCCC(C)(C)O)C)[H])[H]
Target	Endogenous Metabolite,Drug Metabolite,Vitamin
Solubility	DMSO:240 mg/mL (576.04 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:77 mg/mL (184.81 mM)

Bioactivity

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Target IC50	B16 F10 cells:93.88 ppm (0.24 μM)\|Vero cells:431.05 mg/mL\|LNCaP cells:13-23 µM\|267B-1 cells:30 μM\|SUM-229PE cells:14 nM\|BT-474 cells:2.9 nM\|SKBR3 cells:2.4 nM\|C4-2 cells:13-23 µM Read more...
In Vivo	METHODS: To stud the effects on acute lung injury, Calcitriol (1.0 µg/kg) was administered intraperitoneally to CD-1 mice and LPS (1.0 mg/kg) was administered 1-48 h later. Results: Calcitriol significantly attenuated LPS-induced inflammatory cell infiltration in the lungs, as well a the elevation of TNF-α and MIP-2 in serum and lung homogenate. METHODS: To study its effects on atherosclerosis, Calcitriol (20-200 ng/mouse in carboxymethylcellulose) was administered by gavage to ApoE-/- mice twice weekly for twelve weeks. Results: Oral administration of Calcitriol reduced atherosclerotic lesions, macrophage accumulation, and CD4(+) T-cell infiltration in aortic sinuses, and in Calcitriol-treated mice, there was a significant increase in Foxp3(+) regulatory T-cells in mesenteric lymph nodes, spleen, and atherosclerotic lesions and a decrease in CD80(+)CD86(+)DC), as well as a decrease in IL-10 and IL-12 mRNA. -10 and IL-12 mRNA expression was increased. Read more...
In Vitro	METHODS: Malignant pleural mesothelioma cells MSTO-21 h, REN and mesothelial cells MeT-5A were treated with Calcitriol (1-100 nM) for 24 h. Cell viability was detected by MTT Assay. Results: Calcitriol treatment resulted in a dose-dependent decrease in the viability of MSTO-21 h and REN cells the viability of non-malignant MeT-5A mesothelial cells treated with Calcitriol did not change. METHODS: Human colorectal cancer cells HT29 and SW480 were treated with Calcitriol (100 nM) for 48 h, an the expression levels of target proteins were detected by Western Blot. Results: Calcitriol increase the expression of E-cadherin, and decrease the expression of cyclin D1 and c-Myc, two downstream target genes of Wnt/β-catenin signaling in HT29 and SW480 cells. Read more...
Cell Research	Calcitriol is dissolved in DMSO and stored, and then diluted with appropriate medium before us. Experiments are performed using parathyroid tissue slices instead of dispersed parathyroid cells so that tissue architecture is maintained. Parathyroid g and are cut into s All pieces of approximately 1 mM and placed in individual wells (24 well dishes) with constant shaking at 37℃nd placed in an incubator with a humid atmosphere the incubation medium is buffered (pH 7.4) and contained 125 mM NaCl, 5.9 mM KCl, 0.5 mM MgCl2, 1 mM NaH2PO4, and Na2HPO4 (1:2 ratio), 1 mM Na-pyruvate, 4 mM glutamine, 12 mM glucose, and 25 mM HEPES. Insulin 0.1 IU/mL, bovine serum albumin 0.1%, penicillin G 100 IU/mL, and streptomycin 100 μg mL are added to the medium. CaCl2 is added to achieve a final target ionized calcium concentration of 1.25 mM as measured with a Selective electrode (634 Ca/pH analyzer). Ionized calcium concentration is also measured in Each well afte the completion o the experiment, and no change in the ionized calcium concentration is observed. Calcitriol is added to the medium to achieve concentration ranging from 10-10 to 10-7 M. To quantif the amount of free Calcitriol, we filtere the medium through a membrane with a 5000-MW cutoff and Calcitriol is measured by RIA (Gamma 1,25-Dihydroxyvitamin D). As compared with total Calcitriol the concentration of free Calcitriol is decreased by one order of magnitud. Read more...

Storage & Handling

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Storage	keep away from direct sunlight,store under nitrogen,The compound is unstable in solution. Please use soon \| Powder: -20°C for 3 years \| Shipping with blue ice/Shipping at ambient temperature.
Disclaimer	For research use only

Alternative Names

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Inhibitor, EndogenousMetabolite, Endogenous Metabolite, inhibit, Calcitriol, Calcijex, 1,25-Dihydroxyvitamin D3, Topitriol, RO215535, RO-215535, RO 215535, Rocaltrol, Vitamin D, vitamin D receptor, Vitamin D receptor, VD/VDR

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Compound Identifiers

PubChem CID

5280453 ↗

Key Properties

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No computed properties available.

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Reactivity:	All
Dynamic Range:	156.25-10000pg/mL
Sensitivity:	49.5 pg/mL

Reactivity:	Mouse
Dynamic Range:	0.32-20 ng/mL
Sensitivity:	0.129 ng/mL

Reactivity:	Rat
Dynamic Range:	0.63-40 ng/mL
Sensitivity:	0.249 ng/mL

Reactivity:	Gallus
Dynamic Range:	0.63-40 ng/mL
Sensitivity:	0.26 ng/mL

Reactivity:	Human
Dynamic Range:	0.63-40 ng/mL
Sensitivity:	0.257 ng/mL

Calcitriol

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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