Recombinant Calponin Antibody / Rabbit Monoclonal

SKU: orb2642060

Description

Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-3ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
Reactivity	Human, Rat
Application Notes	Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	CNN1/1408R
Immunogen	Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Calponin Antibody / Rabbit Monoclonal

IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of permeabilized human K562 cells with recombinant Calponin antibody (green, clone CNN1/1408R) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human K562 cells with recombinant Calponin antibody (clone CNN1/1408R); Red = isotype control, Blue = recombinant Calponin antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Calponin antibody (clone CNN1/1408R) as confirmation of integrity and purity.

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UniProt

P51911

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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