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Calreticulin Antibody
SKU: orb97537
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Description
Images & Validation
−Item 1 of 5
| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; IHC: 1/100 - 1/500; ICC: 1/50 - 1/500; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | 1G6A7 |
| Immunogen | Synthetic peptide corresponding to aa (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH. |
| Target | Calreticulin |
| Molecular Weight | 48kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Ascitic fluid containing 0.03% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
−RO; CRT; SSA; cC1qR; FLJ26680; CALR
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Western blot analysis of HEK293T cells transfected with the pCMV6-ENTRY control (Lane1) and pCMV6-ENTRY Calreticulin cDNA (Lane2) using Calreticulin antibody
Immunohistochemical analysis of formalin fixed and paraffin-embedded human placenta tissues using Calreticulin antibody
Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using Calreticulin antibody
Western blot analysis of Hela (Lane1), A549 (Lane2), NTERA2 (Lane3) and MCF-7 (Lane4) cell lysate using Calreticulin antibody
Confocal immunofluorescence analysis of 3T3-L1 cells using Calreticulin antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
Calreticulin Antibody (orb97537)
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