Queen's Award Received in 2021 ISO 9001 Certified Delivered over 1,000,000 bio-reagents to life science researchers Trusted by Life Science Communities
Cart summary

You have no items in your shopping cart.

Camptothecin

SKU: orb1309690
FeaturedFeatured Product

Description

Camptothecin is a natural alkaloid and potent topoisomerase I inhibitor (IC50=679 nM) with established antitumor activity. It is widely used in cancer research to induce apoptosis in both in vitro cell studies and in vivo tumor models.

Research Area

Cell Biology

Images & Validation

−
Camptothecin

Key Properties

−
CAS Number7689-03-4
MW348.4
Purity>98%
FormulaC20H16N2O4
SMILESC(C)[C@]1(O)C2=C(C(=O)N3C(=C2)C=4C(C3)=CC=5C(N4)=CC=CC5)COC1=O
TargetTopoisomerase
SolubilitySoluble in DMSO (up to 5 mg/ml).

Bioactivity

−
Target IC50
HCC1419 cells:0.067 µM|HCC1428 cells:0.448 µM|HCC202 cells:0.481 µM|MDA453 cells:0.058 µM|Topo I:0.68 μM|BT-549 cells:0.056 µM|MCF-7 cells:0.089 µM|MDA231 cells:0.04 µM|Sum149 cells:0.065 µM
Read more...
In Vivo
METHODS: To assay antitumor activity in vivo, Camptothecin (0.15-1.2 mg/kg) and doxorubicin (0.25-2 mg/kg) were intravenously injected into athymic nu/n mice bearing triple-negative mammary carcinoma tumors, MDA-MB-231, every two days for four administrations. RESULTS: A dose-dependent reduction in tumor growth was observed, with a 40.8% reduction at 0.5 mg/kg DOX + 0.3 mg/kg CPT and a 93% reduction at 1.5 mg/kg DOX + 0.9 mg/kg CPT. The highest dose tested (2 mg/kg DOX + 1.2 mg/kg CPT) completely stopped tumor growth on day 44. METHODS: To study the effects on obesity, Camptothecin (1 mg/kg, 0.1% Tween 80) was administered orally to obese mice once daily for three days. RESULTS: Oral administration of Camptothecin increased circulating GDF15 levels in diet-induced obese (DIO) mice and genetic ob/ob mice. Consistent with the anorectic effects of GDF15, Camptothecin inhibited food intake, thereby reducing body weight, blood glucose, and liver fat content in obese mice.
Read more...
In Vitro
METHODS: Eight TNBC cell lines were treated with Camptothecin (0.1-5 µM) for 72 h. Cell viability was measured by PrestoBlue. RESULTS: Camptothecin inhibited the cell viability of MCF7, HCC1428, HCC1419, HCC202, MDA453, MDA231, Sum149, and BT549 cells, with IC50 values of 0.089/0.448/0.067/0.481/0.058/0.040/0.065/ 0.056 µM.. METHODS: Lung cancer cells H1299 and H460 were treated with Camptothecin (0.5-5 µM) for 16 h. Cell migration was detected by wound-healing assay. RESULTS: Camptothecin inhibited the migration of H1299 and H460 cells without a dose-dependent effect.
Read more...
Cell Research
Tumor cells are plated in 100 μL of medium in 96-well microtiter plates at a density of 1500 to 4000 cells per well and allowed to adhere overnight. Cells are incubated with Camptothecin for 48 hours and then with fresh medium for 48 hours. Camptothecin at each concentration is added in quadruplicate. Following a 4-hour incubation of treated cells with MTT, the reduced dye product is extracted from the cells with 0.2 mL of DMSO followed by 50 μL of Sorensen's buffer. The plates are shaken briefly, and the absorbance at 570 nm is read and quantitated. Curves are fitted to the MTT assay data using a four-parameter logistic equation.(Only for Reference)
Read more...

Storage & Handling

−
Storage-20°C
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

−
CPT, DNA topoisomerase I, (S)-(+)-Camptothecin, ADC Cytotoxin, alkaloid, ADCCytotoxin, Apoptosis, Antibiotic, Camptothecin, Campathecin, Fungal, HIF-1α, NSC100880, NSC-100880, NSC 100880, InfluenzaVirus, Influenza Virus, microRNAs, MicroRNA, miRN, miRNA, Topoisomerase, Topo I, Top1

Similar Products

−
  • Irinotecan

    Irinotecan [orb1300959]

    >99.99% (May vary between batches)

    97682-44-5

    586.68

    C33H38N4O6

    1 ml x 10 mM (in DMSO), 200 mg, 10 mg, 25 mg, 50 mg, 100 mg, 500 mg
    1. 9-Aminocamptothecin Hydrochloride
    Item 1 of 1

    9-Aminocamptothecin Hydrochloride [orb610601]

    ≥98%

    91421-43-1 (free base)

    399.83

    50 mg, 25 mg, 1 mg, 10 mg
    1. Camptothecins Standards Kit
    Item 1 of 1

    Camptothecins Standards Kit [orb322047]

    1 kit
  • (5R)-Dimethylbenzene-camptothecin

    (5R)-Dimethylbenzene-camptothecin [orb3028879]

    50 mg, 10 mg
  • Pegamotecan

    Pegamotecan [orb1702960]

    97.83% (May vary between batches)

    581079-18-7

    980.984

    C52H48N6O14

    1 mg, 25 mg, 5 mg, 10 mg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID
24360 ↗

Key Properties

−
No computed properties available.

Documents Download

Datasheet
Product Information
Download

Request a Document

Protocol Information

Find More Protocols

Camptothecin (orb1309690)

  • Star
  • Star
  • Star
  • Star
  • Star
  • 0.0
Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

Login to Submit a Review

No reviews yet

Login to review

Step 1: Enter information below

(Recommended: An additional animal making an allowance for loss during the experiment)

Step 2: Enter the in vivo formulation

(This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

100 mg
$ 160.00
1 g
$ 380.00
DispatchUsually dispatched within 3-5 working days
Bulk Enquiry
Need technical support? Find your Distributor Return and Tax Policy

Email

info@biorbyt.com

UK Office

+44 (0)1223 859353

US Office

+1 (415) 906-5211

CN Office

+86 (27)-87663101

Distributors

biorbyt.com/distributor