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Carbonic Anhydrase I Recombinant Rabbit Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:50-200, IF=1:100-500 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 2H4 |
| Immunogen | Recombinant human Carbonic Anhydrase I (1-200/261aa) |
| Target | CA1 |
| Molecular Weight | 29 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Sample: Lane 1: Large intestine (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Stomach (Mouse) Lysate at 40 ug, Lane 4: Bone (Mouse) Lysate at 40 ug, Lane 5: Spleen (Mouse) Lysate at 40 ug, Lane 6: Small intestine (Mouse) Lysate at 40 ug, Lane 7: Lung (Mouse) Lysate at 40 ug, Lane 8: Cerebrum (Mouse) Lysate at 40 ug, Lane 9: Large intestine (Rat) Lysate at 40 ug, Lane 10: Lung (Rat) Lysate at 40 ug, Lane 11: Stomach (Rat) Lysate at 40 ug, Lane 12: Bone (Rat) Lysate at 40 ug, Lane 5: Spleen (Rat) Lysate at 40 ug, Primary: Anti-Carbonic Anhydrase I (orb704263) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 27 kD.
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Protocol Information
Carbonic Anhydrase I Recombinant Rabbit Monoclonal Antibody (orb704263)
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