Caspase-3 (CASP3) Antibody (Center)

SKU: orb1929150

This product can be custom made

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

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Item 1 of 5

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:2000, IHC-P - 1:10-50, FC - 1:10-50
Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD36818
Immunogen	This Caspase-3 (CASP3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the Central region of human Caspase-3 (CASP3). Antigen Region: 60-90 aa.
Target	CASP3
Molecular Weight	31608 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, CASP3, CPP32

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Western blot analysis of anti-CASP3 Pab in NCI-H460 cell line lysates (35 ug/lane).CASP3 (arrow) was detected using the purified Pab.

Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.

Flow cytometric analysis of NCI-H460 cells using Caspase-3 (CASP3) Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP3 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-CASP3 Antibody (Center) at 1:2000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CASP3

UniProt

P42574

RefSeq (Protein):NP_004337.2, NP_116786.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_004337.2, NP_116786.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Flow Cytometry

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