Caspase-3 Rabbit Polyclonal Antibody

SKU: orb10237

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Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Dilution range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit (80-175/277aa)
Target	CASP3
Molecular Weight	17/32 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.

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Sample: Lane 1: Raji (Human) Cell Lysate at 30 ug, Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 3: Lung (Mouse) Lysate at 40 ug, Lane 4: Lung (Rat) Lysate at 40 ug, Primary: Anti-Caspase-3 (orb10237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 37 kD.

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Lymph node (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Mouse) Lysate at 40 ug, Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 6: Spleen (Rat) Lysate at 40 ug, Lane 7: Lung (Rat) Lysate at 40 ug, Lane 8: Lymph node (Rat) Lysate at 40 ug, Lane 9: Cerebrum (Rat) Lysate at 40 ug, Lane 10: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Caspase-3 (orb10237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 37 kD.

Tissue/cell: NIH/3T3 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Caspase-3) polyclonal Antibody, Unconjugated (orb10237) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.