Caspase-9 (phospho Tyr153) rabbit pAb Antibody

SKU: orb770616

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Description

Rabbit polyclonal antibody to Caspase-9 (phospho-Tyr153)

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, WB
Dilution Range	Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Caspase 9 around the phosphorylation site of Tyr153. AA range:119-168
Molecular Weight	46
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-CASP9 antibody, anti-MCH6 antibody, anti-Caspase-9 antibody, anti-CASP-9 antibody, anti-Apoptotic protease Mch-6 antibody, anti-Apoptotic protease-activating factor 3 antibody, anti-APAF-3 antibody, anti-ICE-like apoptotic protease 6 antibody, anti-ICE-LAP6 antibody

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Caspase-9 (phospho Tyr153) rabbit pAb Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Caspase 9 (Phospho-Tyr153) Antibody.

Immunofluorescence analysis of HepG2 cells, using Caspase 9 (Phospho-Tyr153) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COLO205 cells, using Caspase 9 (Phospho-Tyr153) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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