Cattle Interleukin 22 (IL22) ELISA Kit

SKU: orb781855

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 22(IL22).

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL22. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL22. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL22 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Bovine
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	5.9 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ILTIF ELISA Kit, Zcyto18 ELISA Kit, IL-TIF ELISA Kit, IL-D110 ELISA Kit, TIFa ELISA Kit, TIFIL-23 ELISA Kit, IL-22 ELISA Kit, Cytokine Zcyto18 ELISA Kit, IL-10-Related T-Cell-Derived Inducible Factor ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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