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Cattle Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

SKU: orb779316
Free Kit Promotion

Description

The Cattle Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit is designed to quantify Transforming Growth Factor Beta 1 (TGFb1) in bovine serum, platelet-poor plasma, cell culture supernates and other biological fluids. The kit has a detection range of 15.63-1000 pg/mL and a sensitivity of 4.8 pg/mL.

Research Area

Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsELISA
Application Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

ReactivityBovine
Sample Typesserum, platelet-poor plasma, cell culture supernates and other biological fluids
Assay TypeCompetitive
Assay Time2h
Range15.63-1000 pg/mL
Sensitivity4.8 pg/mL
Concentration1000 pg/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

TGF Beta 1,TGF-B1 ELISA Kit, CED ELISA Kit, DPD1 ELISA Kit, LAP ELISA Kit, Camurati-Engelmann Disease ELISA Kit, Latency-associated peptide ELISA Kit
Quality Guarantee

Quality Guarantee

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UniProt Details

No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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48 T
$ 350.00
96 T
$ 490.00
DispatchUsually dispatched within 5-10 working days
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