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CBP 35 rabbit pAb
SKU: orb764756
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Galectin 3. AA range:141-190 |
| Molecular Weight | 27 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-LGALS3 antibody, anti-MAC2 antibody, anti-Galectin-3 antibody, anti-Gal-3 antibody, anti-35 kDa lectin antibody, anti-Carbohydrate-binding protein 35 antibody, anti-CBP 35 antibody, anti-Galactose-specific lectin 3 antibody, anti-Galactoside-binding protein antibody, anti-GALBP antibody, anti-IgE-binding protein antibody, anti-L-31 antibody, anti-Laminin-binding pro antibody
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Immunofluorescence analysis of Hela cell. 1, CBP 35 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Western Blot analysis of various cells using CBP 35 Polyclonal Antibody diluted at 1:2000.
Immunofluorescence analysis of NIH/3T3 cells, using Galectin 3 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Galectin 3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CBP 35 rabbit pAb (orb764756)
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