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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB - 1:2000, FC - 1:25 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This CCL17 antibody is generated from a rabbit immunized with a recombinant protein of human CCL17. |
| Target | CCL17 |
| Molecular Weight | 10507 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−C-C motif chemokine 17, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CCL17, SCYA17, TARC
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CCL17 Rabbit Polyclonal Antibody [orb13289]
ELISA, IF, IHC-Fr, IHC-P
Mouse, Rat
Human
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl
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All lanes: Anti-CCL17 Antibody at 1:2000 dilution. Lane 1: human spleen lysates. Lane 2: human skeletal muscle lysates. Lane 3: human lung lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Jurkat cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
Quick Database Links
Gene Symbol
CCL17
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
CCL17 Antibody (orb1926243)
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